FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_74.351000000398a9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_74.351000000398a9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1388885
Sequences flagged as poor quality0
Sequence length36
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGCA651.6469568E-518.4606974
AGGACTG703.1143947E-517.1420765
GCCCACG550.002102075616.3634820
AGGGGTG1056.754615E-512.8565565
ACAGTGC954.1900057E-412.6310028
GTATTAG5200.011.8263841
CTTATAC2301.05501385E-1011.7385953
GCCCAGA1059.7167265E-411.428051
AGGCAGC1202.3457703E-411.2494876
ACTGGTC1202.3457703E-411.2494878
CTAATAC4950.011.211613
CTAGTAC1901.3252065E-711.0521273
TGTACTG1807.8790436E-710.8328395
CTTACAC1408.1797705E-510.7137983
TATACTG3850.010.5190015
GACAGTG1653.8956146E-59.9995457
TCTGCAC1200.0029556389.9995443
CTAGGAC1200.0029556389.9995443
TAATACT5650.09.822564
CTCCTAT2157.2457806E-79.7669961
Produced by FastQC (version 0.11.2)