FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_73.35100000039865.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_73.35100000039865.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences679631
Sequences flagged as poor quality0
Sequence length36
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTGTA500.001109935617.999167
CCCTACA706.034492E-414.9993022
TATACTA600.00375298114.9993012
TTATATT952.6331682E-514.2098654
TTTATAT851.6325402E-414.1169893
CTACTAA650.00638447913.845511
TATATTC1352.892266E-713.3327123
ATTTATA1056.7448185E-512.8565442
GTATAGA1056.7448185E-512.8565441
CTATACT850.002611278612.35236554
CATCTAT1151.5772643E-411.7385841
TCTATAC900.00400559911.6661223
TCTAGTA1059.705655E-411.4280392
TATTATA1059.705655E-411.4280392
CCTACAC1202.3424397E-411.2494763
TATTCTT1602.2575332E-611.2494765
CTAGACA950.005994412611.0521164
TCTAGAC950.005994412611.0521163
TAGAGGA1100.001431933410.9085834
TCTTAAG1100.001431933410.9085832
Produced by FastQC (version 0.11.2)