FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_72.35100000039822.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_72.35100000039822.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89
Sequences flagged as poor quality0
Sequence length36
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAAAAGATGTGCTCGAGTATTTAACCCACCGAT11.1235955056179776No Hit
GAAATATAGAATTGATCCAGGGTCATCAGTACGATA11.1235955056179776No Hit
ATCAAAATCTACGGGATATAAATCAGCTATACTTTC11.1235955056179776No Hit
CTTATAAACATCTCCGCGCCCACGCGACTAAGGAGT11.1235955056179776No Hit
CACCCACACTGTGCTCCGGCCCGAACTACTCTTGGC11.1235955056179776No Hit
ACAATTAACACCGCCGTCCTCATAATCTCGAATTAC11.1235955056179776No Hit
CTTATATTCCTTCCTTCGTCTCGCCTCGAAGGCAAC11.1235955056179776No Hit
GATGCAGAGGACATGCAAACAGCTCGTTCAATTATT11.1235955056179776No Hit
CAATTTAAAGATCACCATGAAATTGGTGGTACGTCT11.1235955056179776No Hit
ATGTTCCTGTTTGTAAGAACTGTTTAACCTCTACAA11.1235955056179776No Hit
GTCATATGCTTGTCTCAAAGATTAAGCCATGCATGT11.1235955056179776No Hit
TTTTTCATCTGCTCCATCAACTCGGTTCCTCCAATG11.1235955056179776No Hit
GTACGCCATTATCCAGAATGGGTTTGAGGTTGCGCT11.1235955056179776No Hit
CAAGTGGTATGATTCTCGCATTGGGATAAGATAATA11.1235955056179776No Hit
ACTCAAAGCAAAAGATCGTTGTACCATATTAAAAAA11.1235955056179776No Hit
GCTTGTACCATTCATCATTCATTGGTCTGTATTTTA11.1235955056179776No Hit
ATAGGACAATTAAAACCGTTTCAATACCACACACTG11.1235955056179776No Hit
AGCTATAACTTTGGGTTTGGTTGTATTCGTATAGCT11.1235955056179776No Hit
CACAACTTCCGATAAGATTCTTGTGGATGAACTTGA11.1235955056179776No Hit
CTCGCGACAGATCCGCCACGATACTTCTTATGGGGC11.1235955056179776No Hit
ATGAAAGACTTATTGGCTGAAAAGATTGGAAAATAC11.1235955056179776No Hit
TCATTGAACAGGTCCTTAGCAGTTTTATCAGCAAGA11.1235955056179776No Hit
GGTATGAACAGGCCAAAAATTATGAGATTGTTCACT11.1235955056179776No Hit
TACAAAAAAAGGTGCTCAAAGTCAGGCCACATATCC11.1235955056179776No Hit
GAACAATTGATGCGAGATTCTAATAAAAAGCAAGTA11.1235955056179776No Hit
TTCGAAAAGAGTTTGGAAACGAATTCGAGTAGGCTT11.1235955056179776No Hit
TCCGGATCCCGAAACGGAGCCGAAGAAGCTCCCCGA11.1235955056179776No Hit
GTCTAGTAATCTTCAGTTGTTTAGTGGAAGGCTGCT11.1235955056179776No Hit
GATTAGTTCACGAGAAGGGTCCTCTTCAGGCGGTAG11.1235955056179776No Hit
CCATTAGCGCTCGGTGGATTTTGCTGCGGTTGAGGA11.1235955056179776No Hit
AAATGAGAGAGTCCACCAATGGCGCAGCATTTTTGA11.1235955056179776No Hit
TCGTAACCTTCTTCAATAAAGTTTCTTACAGGGGCC11.1235955056179776No Hit
CTTATAAAGTCTTACTAGAATAATTTCTTAAAGTCA11.1235955056179776No Hit
CTCCCTCTCCGGAATCGAACCCTTATTCCCCGTTAC11.1235955056179776No Hit
CTATTCTGGACAAGCAAATTCAAGTACAATAACATG11.1235955056179776No Hit
GAATTTAATAGGCCTTCAAAATTTAAACGTAAAAGA11.1235955056179776No Hit
ATCTTGTTCTCGAGCTTGGGGTCCTTGAGTGGAGAG11.1235955056179776No Hit
ACCGTAGCCGCTGATGTTCTCGAACTCTCTCGCTAT11.1235955056179776No Hit
GACATTTCATTGGATGGACCATATACATAATTGTTA11.1235955056179776No Hit
ATGGTAGTGCTCATACTTCAAGTCAATATGTTTATT11.1235955056179776No Hit
GAGAACAATCCAACGCTTACCGAATTCTGCTTCGGT11.1235955056179776No Hit
GCGTAGTCTCTTGGTGCTCCCGTCGCTGTTGCTCCT11.1235955056179776No Hit
CCGTATGCCAAAAGCATGATACGAGTGACGATTGTC11.1235955056179776No Hit
GCATGAGGACCACCGTAACCAAATGGAACCCCAAAT11.1235955056179776No Hit
GTCAATTCCACTGTTCACTCCACTGTTCACTCCACT11.1235955056179776No Hit
GGGTACACGAGCTGCTCGAAAACGTGACTGGTGACC11.1235955056179776No Hit
AAAACAATCAAAGCTCACAAAAACATGGACGTTCAT11.1235955056179776No Hit
AACTATGACCTGCAACAATAATCTCACCCAATTGAG11.1235955056179776No Hit
CTTTACATTTAGAGCATTACTATATCTCAAAATTTG11.1235955056179776No Hit
ACATATAAAACGGAATGAGGGATAATCGTAATATTA11.1235955056179776No Hit
TCTTGGGCCTTGAGTTTCTTGTGGCTTCTGTGGACT11.1235955056179776No Hit
ATGTCACACCGATTTACATGCTTGGCACGGCGATTG11.1235955056179776No Hit
CAAAACAAACAAAACTGAAAAACAAATAAAAAAACA11.1235955056179776No Hit
TTGCTTCACACTTTCCGGTATTTCAGAAAGCTTTTC11.1235955056179776No Hit
ATGAAAAACTCATATACATTCACCAGATATGTTCTA11.1235955056179776No Hit
TTATGAATCTGTTATGAAAAATCCAATGCAATATCT11.1235955056179776No Hit
AGATAGTAGTAAGAGGAAATGTGCAAAAATGCAGTG11.1235955056179776No Hit
ACTGTAAGATCTTCATTTTTGATACTTTCGCGAGTT11.1235955056179776No Hit
ATATTCCAACGATTGTCGAGGAACAAGACTTACCTC11.1235955056179776No Hit
CCCACACACCACCCTTCCCTAAGCGGTAAAAGATAA11.1235955056179776No Hit
CACAAGATATAATGAATGACACTGTAGCAGAATCCA11.1235955056179776No Hit
CAATAGTGTGTCCGTTTTCGTCTGGGGTGTGGATTA11.1235955056179776No Hit
GACGTAAACAATGTTGTCAGAAGGAAAGATCTCCAT11.1235955056179776No Hit
CCGCTATAGCAGGTAGATTTAAGTCACATTCATCAT11.1235955056179776No Hit
GAAAAAGCCACCGTCATAGTGTAACTGTAATATATA11.1235955056179776No Hit
CCCAAAATGGGACCCCGTAAGATGAAATTAAGTATT11.1235955056179776No Hit
CAAAAGATTAAAATGAATCGAAGAAATCATATATAG11.1235955056179776No Hit
ATACGATACCCCGCATGGAATGGGATAATATCACAG11.1235955056179776No Hit
AATTTTCTACAGATGATTCATTATGTTGCACCCTTG11.1235955056179776No Hit
ACCTAAACCAAGTGGAGATTTTGGATTTCCTTCGGG11.1235955056179776No Hit
CAGTTAATGAATCAAGCGGGCCAAACACACGCTCTC11.1235955056179776No Hit
AGCAGATCCAAGTGAAAGTAGCATTTGGTCATGATA11.1235955056179776No Hit
GACACGCGCCGCTCTACAACAAGCTCCACTAAGCTA11.1235955056179776No Hit
GGCAGATACTACGCCATGAAAGTTTTGAAAAAGGAA11.1235955056179776No Hit
GTGGTGGGATATATGGGAACATTGCCTGACGAGGCT11.1235955056179776No Hit
GCGTCAGATGTGTATAAGAGACAGGCTCCAGCTACA11.1235955056179776No Hit
TACAACAACAACAACAATTTCTTATTACTGTGTCCT11.1235955056179776No Hit
TTTCCAGGCTCTCAATTGGCGTTAATAATTCAATAT11.1235955056179776No Hit
CAACTGTAATTACAAATAAATAAAAAGATATAAAAA11.1235955056179776No Hit
ATACGCATCATCTTGTTCCCAAAACAACCTGAAGCC11.1235955056179776No Hit
TCTTTATGTGCTTGAGAAGAGCCAGTGACAAAGTGG11.1235955056179776No Hit
CTAGAGGACGCATCCTGTCTCAAGTCCAGTAGCAGA11.1235955056179776No Hit
ATACCAGCTAGTACAGCAATTTGTGGACAAATTTAA11.1235955056179776No Hit
TATCTACGTTGTGGGCCTTGCCCTCAACACAAGATT11.1235955056179776No Hit
TGCTCACACCATAAACAAAGGCTGTTAGTTTAGGCA11.1235955056179776No Hit
ATTATACCAAGTAATGAATTGCGAATTCGCGCGAGT11.1235955056179776No Hit
AGATAATAGTAAAGAAGTTACGCTGGAATCTTTAAT11.1235955056179776No Hit
TGCCTGATCAGACAGCCGCAAAAACCAATTATCCGA11.1235955056179776No Hit
GTTATATGGAGAAATTGATACGTTTGCACCAGCAGG11.1235955056179776No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCTCA50.029.99999820
CAAGATT50.029.99999830
GCCTTGC50.029.99999815
ACGTTGT50.029.9999986
CACAAGA50.029.99999828
ATCTACG50.029.9999982
CCTTGCC50.029.99999816
CAACACA50.029.99999825
TCAACAC50.029.99999824
TCTACGT50.029.9999983
TGCCCTC50.029.99999819
ACACAAG50.029.99999827
TACGTTG50.029.9999985
GGCCTTG50.029.99999814
GTGGGCC50.029.99999811
TATCTAC50.029.9999981
CCTCAAC50.029.99999822
CTACGTT50.029.9999984
CTTGCCC50.029.99999817
TGTGGGC50.029.99999810
Produced by FastQC (version 0.11.2)