FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_70.351000000397aa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_70.351000000397aa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences538757
Sequences flagged as poor quality0
Sequence length36
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCGCA400.00650866718.7487269
GGACACT703.1092804E-517.141696
TACACAG703.1092804E-517.141695
ACTATAG706.0326E-414.9989793
AGCCTGG600.003752173414.9989796
ACCGTGC600.003752173414.9989798
GTCTTAT706.0326E-414.9989791
TAGACAG650.006383123313.8452115
TATACAA902.6493776E-413.3324255
CCGCTCG800.001655267613.1241079
TGTAGAC850.002610487212.35213
TAGAGGT900.004004397411.6658734
AGATAGC900.004004397411.6658736
GCTATAT900.004004397411.6658731
GAACCTT1059.7016845E-411.4277946
TATACTG1355.6382436E-511.1103555
GTACTAT950.005992637511.051881
ACTGTTC950.005992637511.051888
TACTATA950.005992637511.051882
CTACTAG1000.0087713510.4992861
Produced by FastQC (version 0.11.2)