FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_67.351000000396d1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_67.351000000396d1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences794791
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTACC653.4362098E-416.1530677
CGGGGCA650.006385803713.8454879
GTAGGGG902.651416E-413.3326893
ACAGATC902.651416E-413.3326898
GTCCAGG954.186598E-412.6309691
GGACTTC850.00261198412.35234456
GGACTGA850.00261198412.35234456
AGACCGT850.00261198412.35234456
TAGGGGT1006.447726E-411.999424
ACACCGA1151.5779336E-411.7385646
ATGCTCT1151.5779336E-411.7385646
AGGACTA900.004006669411.6661035
ACCGTCG900.004006669411.6661038
TATATAC3100.011.6123443
CCTAGTG1059.7089517E-411.428023
TAAGGGT1059.7089517E-411.428024
CTATACT1859.141877E-811.3508034
TACAGTG1202.3434267E-411.2494575
CTTAAGC950.005996001411.0520983
GAGTTAG950.005996001411.0520981
Produced by FastQC (version 0.11.2)