FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_65.3510000003965b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_65.3510000003965b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1776007
Sequences flagged as poor quality0
Sequence length36
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGAC954.191905E-412.630693
AGGACTG1901.0446456E-811.8412715
TAGTACC1653.2765784E-610.9083234
TCAGGAC1253.4255523E-410.7992393
GTATATG4900.010.4074311
TACACTG2053.7778227E-710.2431815
TAGGACT1501.6452909E-49.9992974
ACTGCGC1356.9636136E-49.9992968
GTCCTGA2251.255903E-79.9992961
GTATAGG2609.897121E-99.8070011
TCTAGGA1409.721117E-49.6421792
TATACCG2651.3513272E-89.6219645
ATAATAC4400.09.5447833
CAGGACT2054.1731E-69.5115254
TAGGACA2054.1731E-69.5115254
TTAGACA2403.212008E-79.374344
TCTAGAC2403.212008E-79.374343
TCTTATA3203.4015102E-109.3743392
ATAGGCC1450.00133985779.30968953
CCTACCC1300.00572606739.2301193
Produced by FastQC (version 0.11.2)