FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_64.35100000039617.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_64.35100000039617.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1137050
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCT650.006391577413.8443277
TAGCCTG1251.779008E-613.1982585
CTGGTCA1551.1342672E-712.5789859
ATCACAC1804.654794E-912.50274929
GCTATAC1201.6955128E-512.4983513
TAGACGG850.002614817612.3513125
AGCCCGT850.002614817612.35131211
ACCATGC1151.580309E-411.7375828
GAGTAAC1551.5454061E-611.6113711
GACAGTG1703.7673271E-711.4690757
ACTGAGC1703.7673271E-711.4690758
CTGGACA1703.7673271E-711.4690754
TAGACTG1059.721382E-411.4270655
ACTATGC3350.011.1925538
CAGTCTA950.00600242711.0511739
GTATTAG2351.875378E-910.8496321
CTATGCA3051.8189894E-1210.8182459
CTAGACA1408.183943E-510.71287254
CTAATAC4200.010.71287253
TAATACT6050.010.6597514
Produced by FastQC (version 0.11.2)