FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_63.351000000395d2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_63.351000000395d2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1730702
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG1352.9004514E-713.3329231
TGACGCG800.00165738213.1245957
TATACCG2452.5465852E-1111.6322955
GTATTAG3400.011.0290721
CCTGGAC1501.3607956E-510.9996613
TCCTAGG1100.001433686710.9087542
AGACGGC1807.8818084E-710.8329996
GTCGTAG1551.9561323E-510.6448341
TAGGACT1704.6814075E-610.5879094
CGCGCAT1304.918411E-410.38429610
CCTAAAC1756.6172142E-610.2853983
TAGACGG1901.5794121E-610.2628415
ACTATGC4400.010.2269578
CAAGCCT2251.2550845E-79.9996924
TAGTACT2752.3701432E-99.817884
CTAATAC4600.09.7823073
GACTGTG1851.2814464E-59.7294297
TAGACTG1705.407317E-59.7055845
CTGCGTC1409.7173307E-49.642569
GGGGCTG1409.7173307E-49.642565
Produced by FastQC (version 0.11.2)