FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_62.3510000003959f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_62.3510000003959f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences997561
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGCA650.00638576313.8459929
GGACTGT1004.2698695E-513.4998426
GTATTAG2350.013.40409851
TAGGACT800.001656286913.1248464
CTACCCT1151.0927341E-513.0433254
TGGGGGG850.002612073212.35279755
GTATTAA1951.1641532E-912.30754851
GACTGTT1406.3123716E-611.7855777
CGCCCGT900.004006811.66653112
CTAATAC2605.456968E-1211.5383263
GTGCACT1059.709774E-411.42843811
CCTAATA3300.011.3635032
CCGTCAC1202.3437718E-411.2498699
CACCGCG950.005993948311.05305726
GTACCGC950.005996184411.0525036
CCAAGAC1807.8671474E-710.8332073
TAGCCTA1253.4208532E-410.79987310
CAGAGCG1253.4208532E-410.7998739
TAGATAG1951.893477E-710.7691055
AGGACTG1408.171647E-510.7141615
Produced by FastQC (version 0.11.2)