FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_61.3510000003955c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_61.3510000003955c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1398365
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTAG600.003754581814.99992815
CGGGACA706.043153E-414.998324
TAGGTAG1801.8189894E-1214.9983195
TAGTCAG1903.6379788E-1214.2089349
GATCGGG650.006382622813.84757323
CTCGACT800.001657225513.12446912
CTAGGAC800.001658118113.1235293
TAGGACT1404.5148408E-712.8557024
GTCCTAG954.1923876E-412.6301631
CTAATAC3850.012.4661353
ACTATGC4150.012.287788
GGTAGTC2301.05501385E-1011.7378167
AGACGGC900.004011182611.66535956
TTAGGTA2853.6379788E-1211.0513934
TTAGAAC3150.010.9511553
ATCCTAG1653.2765856E-610.9078691
TCCTACG1100.001434359510.9078692
TAGACGG1408.1853825E-510.7130865
TAGACTG1551.95715E-510.6439685
ACCCTAT1704.683863E-610.58704951
Produced by FastQC (version 0.11.2)