FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_60.35100000039518.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_60.35100000039518.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences632287
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGGGT200.00514088530.00553923
ACGGCAT550.002096538416.36795224
CGGCATA809.719238E-515.00395725
GTCTAAG851.6334384E-414.1157871
TAGGGAC851.6334384E-414.1157879
GGCTTAG800.001656616213.123271
TTAATAC1855.0567905E-1012.9712633
TAATACT3500.012.8554494
ACTATGC1056.748758E-512.8554488
TGTACAG1056.748758E-512.8554485
GTATTAA2255.456968E-1212.6649981
AGGACCA850.002612601312.3513145
CTAATAC2001.7316779E-911.9984193
GCTATAA1151.5781824E-411.7375832
TAGTGCG900.003993432511.67066630
GTGATTA900.004007620711.6651299
CTACACC1059.7109476E-411.4270654
TATACTG2503.8198777E-1111.3984975
TTAAACC1602.2590266E-611.2485174
CTTGTAC950.005997432411.0511743
Produced by FastQC (version 0.11.2)