FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_59.351000000394d3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_59.351000000394d3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1072926
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGCT706.043799E-414.9975774
TAGGACT952.6388307E-514.2082314
GGTAGTC800.001658298713.122887
CAGGACC1056.759169E-512.8550654
CTATGCA1601.7154889E-712.1855329
GTATTAG2001.7389539E-911.9980611
TCCTATA2052.564775E-911.7054262
GACAGTG1951.53359E-811.5365987
GTCTTAC1303.8492784E-511.5365971
CTAATAC2555.4569682E-1111.17466453
GGTGTTC1355.655713E-511.1093168
ACTGTGT1355.655713E-511.1093168
GTATTAT5550.011.079291
CCTAGCG950.00598258911.05599830
TAGGTAG950.0060033811.0508465
GTATAGA2905.456968E-1210.8603141
TTAGGGA1253.426053E-410.7982554
CTCTTAG1253.426053E-410.7982551
GGACAGT1704.6833356E-610.5865256
CACCTAG1000.00875362310.50368828
Produced by FastQC (version 0.11.2)