FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_58.35100000039490.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_58.35100000039490.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences679469
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCCG400.00651123218.7482979
GACAGTG550.002101727816.3621527
CCATGCG706.036514E-414.9986399
GCCACTC600.003754083314.99863913
CTAATAC2050.014.6328173
TACGCTG750.001017891613.9987295
GGCTTAT650.00638634513.8448971
GTCATAG902.6514835E-413.3321221
CCTAATA2400.013.1238092
CATGCGA800.001656326213.12380810
CTACACA850.002612143812.351824
CTCTTAG1101.0425705E-412.2716141
GTATTAG1354.204885E-612.2211141
CTCTTAA1354.204885E-612.2211141
ACTATGC2154.274625E-1011.8593888
TTAGATC1151.5779119E-411.7380654
ACCTAAT2052.5538611E-911.7062541
TCACACA1551.5334354E-611.61697930
AGGACAG1059.709236E-411.4275345
ACGGTGT1602.25873E-611.2489798
Produced by FastQC (version 0.11.2)