FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_57.3510000003945d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_57.3510000003945d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences485123
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGAGT651.644222E-518.4595744
CCCTTAG500.001109759217.9980831
TATTAGG1253.6925485E-1016.798212
GTCCTAA653.434282E-416.1521261
TAGGTAG653.434282E-416.1521265
GGTAGTC859.1816455E-615.88229757
GTATTAG1052.4722794E-715.7126111
GCCTCAC600.003749842314.999948520
TACAGCG600.003749842314.99994857
ACACTCC650.0063791813.84610758
CTATACA1752.0918378E-1013.7128254
GTATAGA1106.8972877E-613.6349121
TAGAAGT1004.26482E-513.4985624
ACTATAC1251.7725033E-613.1985943
CTATGCA1201.6885511E-512.4999579
ACTATGT850.00260857512.3528998
CTAATAC1501.0362055E-611.9987233
GCTTATA1006.442902E-411.9987221
CTATATG1151.5763604E-411.7378814
GTCTAAA1151.5763604E-411.7378811
Produced by FastQC (version 0.11.2)