FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_56.35100000039419.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_56.35100000039419.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences731416
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACT455.465712E-419.99904411
GTACTAG1104.1959902E-714.9992831
GTGTAGG600.003753377414.9992821
GTCTTAC851.6328263E-414.1169721
CTGAGGT902.6509538E-413.3326964
GTCTAGT902.6509538E-413.3326961
ACTGTCC902.6509538E-413.3326968
TGCCGAC800.001656001213.12437312
GTATTAG1404.501453E-712.8565271
ACTGTGC1056.746265E-512.8565268
TACACAG1456.888167E-712.4131985
TTCTAGG1101.0423631E-412.27214052
GTATTAA2001.7316779E-911.9994261
CACAGTG900.004006134311.6661097
TAGGGCA900.004006134311.6661099
CTACACA900.004006134311.6661094
ATAAGCT1059.707303E-411.4280234
GGTTAGA1059.707303E-411.4280231
GTATACG1459.322297E-611.3787651
ATAGGGC1202.342932E-411.2494628
Produced by FastQC (version 0.11.2)