FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_55.351000000393c7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_55.351000000393c7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences992191
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG1451.0913936E-1116.549725
GGGGCTT550.002103069316.36165818
TAGACTG1156.950395E-714.3460915
GCGCACA650.006390353713.8444811
GTCTAAG1201.123346E-613.7483381
TTAGGAC1151.0939506E-513.0419013
GTATAAA4150.013.0104751
GCGTCCT1056.736488E-512.85947624
CTAATAC3600.012.0818723
CGTCGTG1006.432314E-412.00338830
CTATGCA2650.011.8853559
GTAGACC1151.5797596E-411.7377114
GGCTTGC900.004004009511.66760720
CTAAGAC900.004010045411.6652563
TGTAGAC1551.5445457E-611.6114993
ATACTGT3500.011.5700296
TATACTG4450.011.4592885
ACAGGCC1059.718584E-411.4271898
AGTGACC1059.718584E-411.4271898
TAGGACA1459.339226E-611.37793354
Produced by FastQC (version 0.11.2)