FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_54.35100000039384.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_54.35100000039384.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1270101
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGCG402.467401E-422.50041424
TGGGGGG850.002612933312.3526835
CTTACAC1354.2097363E-612.2219663
GTCTTAC1652.5556074E-711.8179331
CCGTCCA1151.5789573E-411.7388849
CTAGCCA900.004008108711.6664224
CTTAGAC1703.7632526E-711.4703483
CGTCGTG1059.7073225E-411.42923230
ATTAGGG1202.3449343E-411.24976353
GGACAGG1202.3449343E-411.24976356
TCTATAC3100.011.12879853
TGTGCGA950.005998131411.05239910
ACCCTGC1501.3598579E-510.9997698
TATACTG4850.010.8245145
TAGACAG1408.176462E-510.7140615
TAATACT5900.010.6777424
GTCCTAC1000.00877929810.499781
CTAATAC3750.010.3997823
GCGAACA1304.9142534E-410.38480621
TCACACA1756.6057546E-610.28630730
Produced by FastQC (version 0.11.2)