FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_53.35100000039341.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_53.35100000039341.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences465843
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATATATATACATGAGAAGAACGGCATAGTGCG7490.16078378337766155No Hit
TATATATACAGGCAACACGCAGATATAGGTGCGACG6440.13824400066116696No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACCCC653.4316914E-416.15354
ATTGCGC653.4316914E-416.153518
GTGCGAC1552.910383E-1115.48520129
TGCGACG1604.9112714E-1115.00128830
ACACCCT600.003749994614.9996786
TTGCGCA809.734137E-514.99967819
GTCCCTA600.003749994614.9996781
TATACTG1950.014.6150715
ATACGGT1156.919763E-714.3475176
ACGGTGT1054.257581E-614.2854088
GGTGCGA1802.1827873E-1114.16788428
GCACTGT750.0010156914.00120329
TATACGG1504.8694346E-913.99975
AGTCCAT650.00637944113.84585710
GTGGACA650.00637944113.8458574
AGACAGC650.00637944113.8458576
TACAGGC1853.45608E-1113.7834887
GTATTAA1201.1184202E-613.7497041
TTATAGT1004.2606996E-513.499714
ATACCTC1004.2606996E-513.499716
Produced by FastQC (version 0.11.2)