FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_52.3510000003931a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_52.3510000003931a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences884847
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAAGC550.002101499416.3632052
GGCCTCA755.6143494E-516.00138719
TAGGTAG1002.5725822E-614.9996055
CCTAGAC650.00638563213.8457893
GAGTCCG650.00638563213.8457899
GTCTACG650.00638563213.8457891
GTATTAG2200.013.6360041
ATAGACC1004.269389E-513.4996448
CTGTGTA1151.0925676E-513.0431349
TACTATG2900.012.9306947
ACTATGC2600.012.6919748
GGTGCGA850.002611959112.35261528
GTTCTAG1601.7111597E-712.187181
TAGAGTG1006.4478116E-411.9996845
AGAGTCC1006.4478116E-411.9996848
GTCCTAA1406.31114E-611.7854031
AGAGTGA1151.5779998E-411.738826
ACTGTGC900.004006626611.6663598
CTAATAC3100.011.61259653
GTATAGA2850.011.5786421
Produced by FastQC (version 0.11.2)