FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_51.351000000392d5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_51.351000000392d5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1478142
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTGCC952.6379368E-514.2095019
GTATTAG4050.013.7027151
TAGGTAG2250.013.3323725
TCGCTCC850.00260964712.35497528
AGTGTGC1006.454808E-411.9991348
CGTGAGC1151.5800449E-411.73828412
GATAGTC900.004010173511.6658257
TCCCCGT1303.848059E-511.5376298
TGTGTCC1059.7195327E-411.4277488
GTACCGT1501.3610474E-510.9992076
GTCCTGC1100.00143396610.9083041
GTGTCCC1408.1827995E-510.7135129
CGTGCAC1000.00878377410.49924210
CCCTAAG1150.002076598810.434031
TTAGGTA3900.010.3838664
TCTACAC2608.967618E-1010.3838663
ACGGCAT2053.7672908E-710.24489624
ACGTGAG1200.00295647989.99927911
ACAGCTC1952.2032746E-69.9992798
ATACTGT5850.09.9992786
Produced by FastQC (version 0.11.2)