FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_50.35100000039292.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_50.35100000039292.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences860213
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCACT400.006510579518.7493111
TAGGTAG1102.3550456E-816.3639855
GACCGTC653.4348012E-416.154197
TAGTCAG1052.476845E-715.7137089
CTATGCA1553.092282E-1115.4833029
ACAGCCT706.033694E-415.00031958
CTAATAC2750.014.182123
GTCTAGC750.00101786313.9994841
CTTACAC1106.90303E-613.6366553
TCACACA1803.274181E-1013.33361730
ATCACAC1803.274181E-1013.33361729
GTATAGA2151.8189894E-1213.2553261
ACCGTCG800.001655552213.1252798
ATCCTGC1006.4449053E-412.0002576
CCTAAGA1006.4449053E-412.0002572
ATACGGT2401.8189894E-1111.8752536
GTATTAG2301.05501385E-1011.7386981
CTAGACA900.00400504611.6669154
CTGTCGT900.00400678611.6662369
ACTATGC2351.5279511E-1011.4896078
Produced by FastQC (version 0.11.2)