FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_49.3510000003926b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_49.3510000003926b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1769045
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCCG1201.1234715E-613.74975411
CTAGCCT1106.9177295E-613.6357354
TGGGGGG902.654248E-413.3327185
GTATTAG3800.013.0260831
GCCCGCT1354.2122283E-612.22200413
GTCTTAT3050.011.3112731
TCTTATA3650.011.0953782
CTAATAC4200.011.0709183
TCCTAGC950.005999568411.0524341
GCACGCC1253.4247705E-410.7995019
GTGTAAG2402.6539055E-910.624811
CTAGGAC1304.919224E-410.3841363
GCCTTAG2209.0367394E-810.227091
CGCTGCG1501.644412E-49.99982216
TAGAGTG2105.255097E-79.9995385
CTGTACC1809.256828E-69.9995384
TAGGTAG2403.003879E-89.9995385
TATTAGG4500.09.9995382
CACGCCC1501.6448811E-49.99953710
TTATACT5900.09.9147964
Produced by FastQC (version 0.11.2)