FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_48.35100000039228.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_48.35100000039228.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences264675
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG505.604852E-827.05
CCTTAGG402.460554E-422.4999982
ACACCCT350.003000112721.4285746
GGTAGTC751.2563032E-720.0000027
TAGACAC400.0064975718.7499985
AATCTAT500.001107156518.06
TTAGGTA859.1498405E-615.8823534
GTATTAG859.1498405E-615.8823531
TCTTATA859.1498405E-615.8823532
GTCAGAT859.1498405E-615.88235311
TAGTCAG859.1498405E-615.8823539
TATTAGG951.4983216E-615.7894742
GTATTAA706.015124E-415.0000011
CAAGGCT706.015124E-415.00000127
ATAAGAT706.015124E-415.0000013
TAGGGAA706.015124E-415.0000015
TTGGACA600.003743760815.04
GCTATAT600.003743760815.01
AGTCAGA809.707103E-514.99999910
TAGACAT809.707103E-514.99999928
Produced by FastQC (version 0.11.2)