FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_47.351000000391e3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_47.351000000391e3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences519781
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTAGG402.4685208E-422.4952372
GGTAGTC755.621529E-515.99661357
AGACCTC600.0037555214.9968265
GTATTAG1156.9315865E-714.34616851
TCCTATA650.00638879713.8432242
ACATGTC650.00638879713.8432248
TGCGTAA650.00638879713.84322410
TATTAGG1352.8936483E-713.3305122
TTAGGTA1251.7752955E-613.19720654
TAGTCAG800.001656921913.1222229
ATTAGGT1404.5019806E-712.8544223
GCGTGTT1056.7493136E-512.85442111
TTAGGTT850.002613086512.3503274
TAGGTAG850.002613086512.3503275
GTAGTCA850.002613086512.3503278
AGGTAGT900.0040083711.6641986
GCCTGTG900.0040083711.6641989
ACTGGTC900.0040083711.6641988
ACTAGAC1202.3440098E-411.247623
GTTTAGA1355.640074E-511.1098291
Produced by FastQC (version 0.11.2)