FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_46.351000000391a0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_46.351000000391a0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences356109
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATAC1052.4662586E-715.7129633
ATTAGAC600.00374924914.9987373
TAGACTG600.00374924914.9987375
ATAGTGT851.6296186E-414.1164586
ACCTATA650.006378204613.8449871
CCAAGAC850.00260780612.3519013
CTCTATA850.00260780612.3519011
CTTAGAC850.00260780612.3519013
GTACAGT1101.0398205E-412.2716921
ATCTTAT1101.0398205E-412.2716921
AACAATC1006.434283E-411.9989897
GTATTAA1252.5641593E-511.9989891
GGTTTAT1006.434283E-411.9989891
GTATACG1006.434283E-411.9989891
CAAGACA1006.434283E-411.9989894
ATTATGC1006.434283E-411.9989898
GTTGTAT900.004000323411.6656851
CCTAATA1303.8294434E-511.5374892
TTATACA1355.6267414E-511.1101754
TATTAAC1100.001429481910.9081722
Produced by FastQC (version 0.11.2)