FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_45.3510000003916c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_45.3510000003916c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences243741
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAAG402.4597772E-422.5000021
CTCTATG559.539968E-519.0909081
GGTATAT400.00649612518.7500021
TAAGATA400.00649612518.7500024
AGTGTCT400.00649612518.7500028
GTATTGG653.4227388E-416.1538471
TCACACA653.4227388E-416.15384730
CTTCTAT653.4227388E-416.1538471
TCTATAC859.143889E-615.8823533
TACTATG809.702077E-515.0000017
CTATACT706.0127024E-415.04
TAGTACC600.003742627815.04
ACTATGC750.001013908414.08
ATAACCT650.00636703213.8461545
TATAAGA650.00636703213.8461542
AATACTA1004.2435342E-513.5000015
CTATGCA800.001649896713.1250019
TATACTG800.001649896713.1250015
TATAGAA954.1664252E-412.6315792
TTATACT1201.6803571E-512.54
Produced by FastQC (version 0.11.2)