FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_44.3510000003911c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_44.3510000003911c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences388870
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAT350.003003141821.4279291
ATACCCT400.006504058418.7494374
GACTAGG752.7873393E-617.999468
CTTAAAC601.8621015E-417.4994753
AGACTAG805.1568622E-616.8744957
ACTAGGC805.1568622E-616.8744959
CTAGGCA805.1568622E-616.87449510
ACGTGAG550.002098701216.36314611
CCTAGCG859.152212E-615.885962530
ACCTAGC859.163394E-615.88392129
CGATCAC859.174586E-615.88187724
ACCTAAT1052.4664587E-715.7138141
TCACCTA1104.1743806E-715.00147927
CACCTAG901.5747419E-515.00147928
CGTGAGC600.003748722614.99955112
GTCTTAG600.003748722614.9995511
TAGAGAC901.5766593E-514.999554
TATTATA1801.8189894E-1214.999552
GTCTTGT706.0255086E-414.999551
AGAGACT901.5766593E-514.999555
Produced by FastQC (version 0.11.2)