FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_43.351000000390d7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_43.351000000390d7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences299269
Sequences flagged as poor quality0
Sequence length36
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCC200.00514208829.9989979
TGTATAC300.001224673924.9991633
TAGTACA350.003001602421.4278554
CAGCGCA400.006500764318.7493729
ACACCGT400.006500764318.7493726
ATTACAT400.006500764318.7493723
CTCTTAT400.006500764318.7493721
AACACTA400.006500764318.7493725
CAGCGTG601.8603409E-417.4994149
TATACAG908.5333886E-716.666115
TAACACT550.00209722816.3630894
TAGACAG653.4268777E-416.1533075
CTATACA653.4268777E-416.1533074
TTCTATA859.160754E-615.8818222
TTGTCAC600.003746119314.99949810
CACTGTG706.019913E-414.9994987
AGAACCG600.003746119314.9994985
TACATAG600.003746119314.9994985
TTAAGAG706.019913E-414.9994983
TCTAAGA901.574299E-514.9994982
Produced by FastQC (version 0.11.2)