FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_42.35100000039094.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_42.35100000039094.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences804753
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGTCT550.002101110816.36348
GATCCGA706.0347345E-414.999783521
GTAGCAC809.747576E-514.9997833
GTGTAAG1301.12522685E-814.9997831
CTCCTAA809.747576E-514.9997831
AGGAGTC600.00375298114.9997827
TCTAGGG650.006384469613.8459532
TAGGGCA650.006384469613.8459534
GTCTAAG850.002611372412.3527621
CTAGACA1006.4460526E-411.99982554
CCTACAG900.00400573311.6664973
CTGGACT900.00400573311.6664974
TATACCG1551.5413698E-611.6127355
GTTCTAT2103.7289283E-911.4284061
TTCTATA3450.011.3041842
GTATTAA2002.2204404E-811.2498371
TAAACCG1202.3427632E-411.2498365
TAGGTAG950.00599460411.0524715
GTACTGG1501.3580457E-510.999841
TACACTC1501.3580457E-510.999845
Produced by FastQC (version 0.11.2)