FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_41.35100000039051.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_41.35100000039051.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences674645
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCTA400.00650907318.7493525
GGACCAT653.4348908E-416.1532866
CTGTGTG653.4348908E-416.1532869
ACAGCCG809.7455275E-514.9994818
GTATAGC901.5801455E-514.9994811
GTATAGA1251.1308293E-714.3995021
TAGTTGC650.006383909413.84567416
ACAGGGC650.006383909413.8456748
GTACTAC1004.2667747E-513.4995341
TAGACTT800.001655604313.1245465
CTCTTAT1151.0917451E-513.0430271
CATGCAC1006.444758E-411.9995853
TCTTATA1151.5770557E-411.7387252
GTTACAC1151.5770557E-411.7387253
ATAGACC1551.540524E-611.6125023
GTATTAG1703.7538848E-711.4701911
CATAGAC1059.7045145E-411.4281773
ATAAGAC1059.7045145E-411.4281773
TACAGCG1059.7045145E-411.4281777
CTTAGAC1059.7045145E-411.4281773
Produced by FastQC (version 0.11.2)