FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_40.3510000003901d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_40.3510000003901d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences305062
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGAC200.005143325329.9977054
TGCGACG908.367351E-1121.6685630
GTATTAA951.70985E-1020.5247461
AAGTGTA455.4567406E-419.998477
CTAACCC400.006502662318.7485663
GAGCCAC400.006502662318.74856611
GGTGCGA1056.548362E-1018.57305328
GTGCGAC1056.548362E-1018.57305329
TACCGAG550.002097956616.3623857
CGCAGAT1306.4028427E-1016.1552618
CAACACG1154.0450686E-815.65097713
AGGTGCG1256.6374923E-915.60136427
ACGCAGA1401.8280844E-915.00131117
TAGGTGC1401.8280844E-915.00131126
ATAGCTC600.00374741314.9988546
TATTAAC1002.5605405E-614.9988532
TACAGGC1351.8368155E-814.4433397
AGGCAAC1251.12448106E-714.39889810
CAGTCTC750.001015524613.9989299
ACACGCA1301.8119863E-713.84736515
Produced by FastQC (version 0.11.2)