FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_39.35100000038fdb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_39.35100000038fdb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences559021
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCA455.465142E-419.9981529
GTCTAGA601.8648323E-417.4983831
CTTGCGC601.8648323E-417.49838310
TAGGTAG1001.420376E-716.4984745
CTACCCC653.435085E-416.1523534
GTAGTCA1052.473862E-715.7128338
GCTCGCG706.0342194E-414.99861312
TAGTCAG901.5800473E-514.9986139
CCCTTGC952.632657E-514.2092138
TACCCCT851.6323575E-414.1163425
GTCCTAT851.6323575E-414.1163421
ATTAGGT2150.013.9521993
CCCCTTG650.006384547313.8448747
ACTGAGT650.006384547313.8448748
GAGTATA1301.8251922E-713.8448741
CTAAGCA650.006384547313.8448744
TTGCGCT650.006384547313.84487411
TTAGGTA2250.013.3321014
TATTAGG2001.2732926E-1012.7488212
AGTCAGA1302.7540464E-612.69113410
Produced by FastQC (version 0.11.2)