FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_38.35100000038f98.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_38.35100000038f98.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences622956
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGGA300.001226363724.9984639
CTAAGGG500.001109905917.9988923
TAGCCTA750.001017499813.9991395
GTTTAGA800.001655692513.1241921
TACACTC800.001655692513.1241925
CCATACT1056.744006E-512.8563524
CTTAGCC1201.6913824E-512.4992313
TTAGCCT1252.5708177E-511.9992614
CTGTTTA1151.57708E-411.7384089
AATAGGG900.004005403311.6659492
AAGGTGT1551.5403966E-611.6121896
GTTTAAT2206.2573235E-1011.5901961
TAAGGTG950.0059941311.0519515
TACTAGC950.0059941311.0519512
GTACAAT1653.265095E-610.908421
ATAACTA1408.164448E-510.7136265
CAATACT2251.0953954E-810.666014
GTATAAA2002.6782618E-710.4993531
TATAGTG1150.002073517510.434145
CTCTACA1304.910335E-410.3839772
Produced by FastQC (version 0.11.2)