FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_37.35100000038f55.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_37.35100000038f55.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences890140
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGAG902.6526576E-413.3322855
TATTATA3800.012.2358812
GTCTAGA1151.5787884E-411.7382071
GTCTTAG900.00400822411.665751
ATAATAC4000.011.6240853
GTATTAG2351.5279511E-1011.4884591
CTATTAT3500.011.1419811
ACAGGGC1100.001433072110.9082348
TAGGGTT1000.00877958910.4991735
TATAATA5150.010.48461152
GTATAAC1304.9155485E-410.3837991
GTATTAA2752.1464075E-1010.3628221
GTCTAAT1756.60932E-610.2849051
TAATACC2505.18412E-910.1991984
TTAGGGT1653.8927537E-59.9992144
GCCTTAC1200.00295466869.9992131
ATACAGG2003.040117E-69.7492326
ACCACTC1705.402173E-59.7051198
CTGCTCA1409.711796E-49.6420989
CAGGACT1250.00414138859.5992454
Produced by FastQC (version 0.11.2)