FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_36.35100000038f2e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_36.35100000038f2e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences465903
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGCCC400.00650643418.74919510
TACCCTG850.00260908112.3524115
ACAGTCC900.004002255411.6661668
TAACACT900.004002255411.6661664
GAGTAGT950.005989458411.0521561
CAGCTAA1100.001429142810.90979421
ACACTAT1253.414454E-410.7995366
TGAACTG1150.002071509610.4343345
ATAAGAG1150.002071509610.4343343
ACTAATA1451.16351606E-410.3443832
TTACTCT1602.7654954E-510.3120574
TAGTACC1756.5847053E-610.2852744
CTCTTAT1356.941372E-49.9995711
TTCTGTA1501.6389301E-49.9995712
TATCCTT1653.880406E-59.9995715
TTATATA2003.0271713E-69.7495812
GTATTTG1409.6902857E-49.6424441
TTAATCT1250.00413390449.5995874
TTTAATC1250.00413390449.5995873
ATACCCT1250.00413390449.5995874
Produced by FastQC (version 0.11.2)