FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_35.35100000038ee9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_35.35100000038ee9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1760244
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGGCC1507.3949195E-812.9991768
TAATGCG1000.00878431910.4993345
GCAGGCC1304.9199804E-410.3839578
GGCTGAG1356.9631037E-49.9993667
TCTTATA5150.09.9022852
GTCCTAT1851.2819808E-59.7291131
GTCTAAT3254.5474735E-119.6916931
GTCTATG2352.3657776E-79.5738611
GTATATA7700.09.5448491
GTATTAG3705.456968E-129.3237331
TATACTG4700.09.2547325
GTATAAA5250.09.1422781
ACTGTGT2157.64102E-69.0691928
GTCTTAT3654.7293724E-119.0405231
GTATTAA5650.09.0259761
AACGCTG1500.00182476158.999435
GTTATAA3358.112693E-108.9546561
TAGACAG2854.4148692E-88.9468015
AGAACCG1705.7737733E-48.8229695
AGTGTGC2054.305776E-58.7799318
Produced by FastQC (version 0.11.2)