FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_34.35100000038ea6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_34.35100000038ea6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences746954
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTACG400.006511681218.748371
AGTGTCG550.00210193916.3622118
AGGATCG650.006386969213.8449495
ATTATAC2503.6379788E-1211.9989563
TATTATA3750.011.598992
ACTGAGC1303.842703E-511.5374578
CTAGATA1355.6460995E-511.1101444
GTATTGG2701.2732926E-1111.1101441
GTCCTAA950.005997160411.051671
CAGTCTG950.005997160411.051679
TATTAGG1501.3587751E-510.99904252
GGTATAA1807.86471E-710.832391
CTCTATG1408.1715734E-510.7133522
GTGTAAG1704.6729856E-610.5873141
ATAGAGC2156.4355845E-810.4642066
ATCTAGG1150.002074787910.4338741
GTATAAT2651.2423698E-910.1877931
TCTTAGG1200.00295392359.999132
TCTAGTA1653.890832E-59.9991292
TTATACT4050.09.9991294
Produced by FastQC (version 0.11.2)