FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_33.35100000038e62.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_33.35100000038e62.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences274210
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTAAT559.545512E-519.090911
TAATACA901.5728463E-514.9999994
TGCTAGA650.006369716513.8461532
TATTATA902.6404322E-413.3333332
CTTATAC800.001650814413.1250013
TATACCT1056.713401E-512.8571424
ACCATTC1006.421417E-412.08
GTCTTAT1006.421417E-412.01
TATACTG1406.264865E-611.7857145
CTCTATA900.00399379811.6666662
ATAATAC1059.669638E-411.4285713
TTATACT1459.25825E-611.3793114
GTATATA1653.240977E-610.9090911
GTTTATA1000.00874850510.51
TATATGT1000.00874850510.54
ACTTACC1000.00874850510.58
TATACTT1409.6596364E-49.6428585
TAATATA1250.0041232689.62
TATACAG1250.0041232689.65
ATATACT1450.00133144229.3103454
Produced by FastQC (version 0.11.2)