FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_32.35100000038e2f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_32.35100000038e2f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences664683
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACTG350.00300592921.4272275
CTGATCG504.601105E-520.9986849
TAGGTCG400.00651003118.7488235
ACCGTGC400.00651003118.7488238
GGAGACC550.002101268916.362613
TTAGACT1052.4750807E-715.7133024
TACCCTT1301.12468115E-814.9990595
TCACACC809.747518E-514.9990593
GTCCCTA809.747518E-514.9990591
CCCTTAT1156.935861E-714.3469271
GTCTTAT851.6326786E-414.1167621
TATACCG1201.1210086E-613.7491385
GTATTAG1106.9033704E-613.6355091
TAGACCG1106.9033704E-613.6355095
CTACCCT902.650717E-413.3324974
ACCTAAT1251.7744514E-613.1991721
TGGACTG800.0016559113.1241765
TACAGGC954.185505E-412.6307887
AATACTA2153.274181E-1112.5573515
ACCGTCG1201.6918053E-512.4992178
Produced by FastQC (version 0.11.2)