FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_31.35100000038df7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_31.35100000038df7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1533932
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCG400.00651473318.7483719
ACAGGGG650.00639101213.844958
ACGGTCC650.00639101213.844958
GACTGTG1454.7451977E-813.4471067
GAGTCCG800.001657931713.1238597
ACCGTGT800.001657931713.1238598
TAGCCTA1101.0441843E-412.27166110
AGACTGT1806.272603E-811.6656526
ACTATGC3750.011.5989918
CCTTAGG1459.344969E-611.3783212
TAATACT6350.011.3375984
TAGGACA2259.131327E-1011.3323484
CGCCGCG1202.3413006E-411.25195724
TATACTG7200.011.2490225
GTATTAG4300.010.8133661
GTATATA9900.010.7569911
TTAGGGG1408.184486E-510.7133543
CTAATAC4950.010.6051393
CCTAATA4550.010.5485332
ACCTAAT4850.010.5148931
Produced by FastQC (version 0.11.2)