FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_30.35100000038db3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_30.35100000038db3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1676681
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCAGA1701.3278623E-1014.1172971
CCGTGCC650.006388711313.845819
GCGTCAG1151.093938E-513.0431544
CTAATAC5900.012.4573173
CTAGACA2051.9463187E-1012.4387154
GCCGTGC850.002613610412.3526348
ACTATGC4100.011.7070268
CCGTGCA900.00400913811.6663789
GCTCTAG1806.268783E-811.6663781
GAGCGGA900.00400913811.66637811
GTGCTCC1059.716873E-411.42828759
TACACAG3200.010.7809835
GGGTAGG1851.1212869E-610.5402781
GTATTAG4450.010.4491791
TAGGTAG2453.7271093E-910.4079055
GTATACC2905.2750693E-1110.344574
GTTTAGG1602.776704E-510.3122441
TAAGGGT2803.0013325E-1010.1783194
TAATACT8550.010.1751864
CCCTAAG1501.6444107E-49.9997521
Produced by FastQC (version 0.11.2)