FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_29.35100000038d70.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_29.35100000038d70.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1597478
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGAC1454.721187E-813.45238929
GTCCTAC800.001658162913.1236721
CTAGACT1151.580625E-411.7379434
GACTAGG1151.580625E-411.7379438
GTATAGA4350.011.7229521
GACAGTG2301.3151293E-911.08583457
CAGTGCG950.00600285711.0515149
CTAGGAC950.00600285711.0515143
GTATTAG4750.011.0515131
GGTGCGA1501.3555464E-511.00336528
GTATTAC4100.010.97451
TGCGACG1408.152412E-510.71756230
CTATGCA2802.7284841E-1110.71320159
CTAGCCT1408.186164E-510.71320154
TAGGACA1851.1222619E-610.5394744
TAGGACT1000.00878618510.4989374
CCTGCTA1602.7788186E-510.3114578
CTAGGCA1602.7788186E-510.31145710
ATCCTAG1756.621738E-610.2846731
CAGTGCA2053.7783866E-710.2428669
Produced by FastQC (version 0.11.2)