FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_28.35100000038d3d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_28.35100000038d3d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1363337
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG1004.2752334E-513.49879555
AGACGGC1201.6953534E-512.4988846
TATACGG3450.012.1728265
CAGGGCT1151.5801235E-411.7380834
TGCGCCA1151.5801235E-411.73808310
GCGCCAA900.00401041211.66562611
GTGCGCC900.00401041211.6656269
TACCGCG900.00401041211.6656267
GTATTAA4800.011.5614681
ACAGCTC1303.848219E-511.5374328
GTAGACG1202.3466599E-411.2489964
ACGGTGT2701.2732926E-1111.110128
TACGGTG2853.6379788E-1211.0516457
GTATTAG3750.010.7990361
TCTAAGC2002.689485E-710.4990633
ATACGGT3450.010.433856
CTGTCCC1304.919626E-410.3836889
ACTGTCC1304.919626E-410.3836888
TGGACTG1602.7776356E-510.311585
TCTGGAC2105.2543874E-79.9991083
Produced by FastQC (version 0.11.2)