FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_27.35100000038d06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_27.35100000038d06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1706650
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGGG350.003008329521.4266262
TGCGCTC600.003756451714.99907712
GCGACGG851.631374E-414.120501529
TAGACAG3150.011.9036825
CTCCTAT2154.292815E-1011.8593871
GTCCCCA1059.7200135E-411.42786911
GGTAGTC2351.875378E-910.8503957
CTAGTAC1807.8876474E-710.832353
ATACGGT4450.010.7858536
ACGGTGT3350.010.7456078
GTCCAGG1408.186057E-510.7133131
CGGTGTT2957.2759576E-1210.6773099
GTATTAG4250.010.5872741
GCCGCGT1000.00876685610.50212425
AGTGCCC1000.00878401110.4993548
TCCTATA4150.010.4809762
TACGGTG4200.010.3565067
TATACGG4350.010.3438885
CTAATAC4850.010.2052593
GTTCTAA3700.010.1342141
Produced by FastQC (version 0.11.2)