FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_26.35100000038cc1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_26.35100000038cc1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1163386
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAGTC1850.016.2144057
TCCGGCC600.00375674914.99832312
TAGGTAG1950.014.6137515
GTATTAG3450.014.3462231
TAGACTA750.00101881913.9984355
TAGTCAG2200.013.6348399
CTAGCCT1106.9175476E-613.6348394
CTAAGAC1352.9005423E-713.3318433
CAGCGCC800.001657822813.1235349
TATTAGG3750.012.3986142
AGTCCGG850.002614485312.35156110
TCTAAGC1702.8303475E-812.3515613
GCCTCAC1101.0419151E-412.27399320
ACGTGAG1354.2131487E-612.22085711
TATACCG1754.2382453E-811.9986595
CTCCTGG1501.0404183E-611.9986581
ATCACAC2553.6379788E-1211.76743729
TACGGAC900.004010480411.66536313
CTAAGCA1703.766636E-711.4693064
ACACCGT1459.342128E-611.3780396
Produced by FastQC (version 0.11.2)