FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_25.35100000038c8e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_25.35100000038c8e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences555750
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGTT200.00514892229.99532112
TAGTCAG801.1041266E-820.6217849
TAGGTAG1155.456968E-1219.5621665
TATTAGG1600.018.7470762
ATTAGGT1252.0008883E-1117.9971923
TTAGGTA1202.0736479E-1017.497274
AGACTAG550.002101996216.3610847
GCTATAC600.003754565314.9976611
GTATTAG1503.0377123E-1014.997661
GGTAGTC1453.0304363E-914.48057
GTATAGA1156.9372436E-714.3455891
TAGAACC952.63418E-514.2083094
ATAACCC750.001017983513.9978163
GTCCAGA750.001017983513.9978161
GGTAGCC750.001017983513.9978168
GTGTAAG650.00638717313.8439941
TAATACT3000.012.9979724
GTAGCCA954.1868808E-412.6296089
CGGATCT850.002601826812.35768629
GCCTCGT850.00261062512.35212719
Produced by FastQC (version 0.11.2)