FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_24.35100000038c4b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_24.35100000038c4b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences600932
Sequences flagged as poor quality0
Sequence length36
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTCCG559.577808E-519.0887914
CGGTCGG400.00651113518.7479213
AAGCGGG500.001106497118.00699230
CTGAGGG1102.3546818E-816.361829
TATCCCG653.436105E-416.1520545
CCCGTCA600.003753915714.99833614
CGGCAGG1156.936443E-714.34623422
AGAGTGT1156.936443E-714.3462346
CTAAGCC952.62139E-514.21604626
ATCGTGG851.6329357E-414.116086
AGTGCAC750.001017806213.99844619
CAGCGCA650.00638606413.8446189
GCTATCC1301.8263745E-713.8446183
CCATGCG650.00638606413.8446189
TAGGACT650.00638606413.8446184
TATCCTG1955.456968E-1213.8446185
TCTTATA2200.013.63485052
CTAAGCA1106.9041744E-613.63485054
CTATTAT2101.8189894E-1213.5699231
ATTGGGC1004.268319E-513.4985023
Produced by FastQC (version 0.11.2)