FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_23.35100000038c07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_23.35100000038c07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1510064
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCA4000.013.8727959
GCTCGGT650.006393999313.84395311
GTATTAG4100.013.1690741
CCCTAAG800.001658397513.1233481
CTGAGCG800.00165881113.1229139
ACCTAAT4500.012.9983641
TCCGGGG1006.459657E-411.99809411
CGGCCAC900.00400589711.66751720
CAGCGTG1806.278606E-811.6648139
AGGGGTG900.004012840311.6648135
TAATACT8650.011.6166514
CTAATAC6350.011.5729633
CCTAATA6100.011.555542
TAGGTAG2607.2759576E-1211.5366285
CCCGTCT1303.8515984E-511.53662813
ATCACAC3800.011.45123729
TCCCTAG1202.3477754E-411.2485851
CTAGCCT1100.001435036410.9073564
TTAGGTA3900.010.7675194
TATTAGG3950.010.6312222
Produced by FastQC (version 0.11.2)