FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_22.35100000038bc2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_22.35100000038bc2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences712925
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACTGG908.582683E-716.6645246
CCTAGAC859.204687E-615.880313
TAGGTAG1052.4776818E-715.7122645
CTGTGCA1156.9430644E-714.3459819
TGGACTG1054.269965E-614.2838765
GACTGCT650.006388351313.8443737
TTAGGTA1352.895813E-713.3316194
ACGGTTA800.001656937213.1233136
TAGATAG1753.0668161E-912.855495
TAGTCAG1404.5053275E-712.855499
GTATTAG2255.456968E-1212.6650381
GTATTAC1907.712515E-1012.6299551
GGTAGTC1456.894079E-712.4121977
ACCTCAG850.002608980312.35395222
GTCTTAG1101.0430783E-412.2711491
CTAATAC1857.010385E-912.1605983
TCTTAGA1754.2289685E-811.9984572
TATTAGG1754.2289685E-811.9984572
TGCACTG900.00400838111.6651675
ACTATGC1703.7595964E-711.4691138
Produced by FastQC (version 0.11.2)