FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_21.35100000038b8f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_21.35100000038b8f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119261
Sequences flagged as poor quality0
Sequence length36
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTGA200.005128994629.99999810
ATACTGA200.005128994629.9999986
GTGAGCT200.005128994629.99999813
TACTATG300.001220117625.07
GTTTTAT402.4495664E-422.4999981
ATGCAAA455.427206E-420.00000211
TACACCA455.427206E-420.0000024
GGGAACG400.00647712818.7499987
TAGGGAA500.001102311318.0000025
TATCGTT650.006341913213.8461535
Produced by FastQC (version 0.11.2)